deadspace
Isaac uses an Oxygen Recharge Station on the USG Ishimura.

Isaac uses an Oxygen Recharge Station on the USG Ishimura.

Oxygen is a member of the chalcogen group on the periodic table and is a highly reactive nonmetallic period 2 element that readily forms compounds with almost all of the other elements. Two atoms of the element binded to form dioxygen with the formula O2. Oxygen was the third most abundant element in the universe and the most abundant element by mass in Earth's crust.

There are several sections of the USG Ishimura, USM Valor and Titan Station that are open to the vacuum of space. When Isaac entered one of these places, his suit automatically sealed itself airtight and an Oxygen meter started to count down. If Isaac failed to either get to an Oxygen Recharge Station, a pressurized room or utilize an air can in time, he will suffocate and die.

Upgrading

The amount of oxygen that Isaac can use can only be upgraded by applying power nodes to the RIG's oxygen containment unit, raising it's capacity. Isaac can carry a maximum of two minutes and ten seconds of air in Dead Space, three minutes of air in Dead Space 2 and seven minutes of air in Dead Space 3.

Trivia

Sources

Wikipedia
There is more information available on this subject at Oxygen on the English Wikipedia.